In order to improve the recovery rate of metal resources in a copper and cobalt mine in Hainan, the effects of regrind fineness, collector type and lime dosage on the copper and cobalt mixed flotation process were systematically investigated based on a process mineralogical study of the raw ore. The results show that the grades of copper and cobalt in the raw ore are 1.28% and 0.44%, respectively, and the copper and cobalt mainly exist in the sulfide mineral phase, in which the average embedded particle size of chalcopyrite is about 0.15 mm, and the average embedded particle size of cobalt sulfide is only 0.084 mm. Through the optimisation experiments of the flotation parameters, it was finally determined that the copper and cobalt mixed flotation was carried out under the conditions of grinding fineness - 325 mesh accounting for 68.28%, lime dosage of 2000 g/t and Z200 as the flotation collector, and then the copper and cobalt separated flotation was carried out, which could obtain the copper concentrate with a grade of 24.90% and a recovery of 83.83%, and the cobalt concentrate with a grade of 1.11% and a recovery of 88.28%. Compared to the previous process conditions, the quality of both copper and cobalt concentrates was significantly improved and the results of this study are of great importance for the efficient utilisation of copper and cobalt ore resources.